Comparison of Monkeypox Virus Clade Kinetics and Pathology within the Prairie Dog Animal Model Using a Serial Sacrifice Study Design
Table 3
Viral kinetics and tissue tropism of MPXV infection within the prairie dog.
Tissue sample
West Africa MPXV
Congo Basin MPXV
DNA presence (days)
Virus presence (days)
Peak mean viral load value and day
DNA presence (days)
Virus presence (days)
Peak mean viral load value and day
Nasal cavity
4–24
6–17
7.7 × 108 Day 9
2–17
4–17
2.6 × 108 Days 12/13
Salivary glands/SM lymph nodes
2–24
6–12
6.8 × 105 Day 6
4–24
4–13
9.9 × 107 Day 6
Spleen
4–24
6–12
4.9 × 108 Day 12
4–17
4–13
7.5 × 108 Days 12/13
Tongue
4–24
6–17
2.1 × 107 Day 12
4–17
6–17
4.8 × 107 Days 12/13
Tonsils
4–24
6–12
7.7 × 107 Day 12
4–17
6–13
4.6 × 106 Days 12/13
Oropharynx
4–24
6–17
4.1 × 106 Day 12
2–24
6–17
2 × 107 Days 12/13
Inner cheek
6–24
6–17
2.4 × 107 Day 12
4–17
6–17
2.8 × 107 Days 12/13
Trachea
6–24
6–12
2.7 × 106 Day 12
4–24
6–13
1 × 107 Day 9
Lungs
4–17
6–12
5.9 × 107 Day 12
4–24
6–13
3.9 × 107 Days 12/13
Liver
4–17
6–12
5.7 × 108 Day 12
2–17
6–12
2.9 × 109 Days 12/13
Ileum
4–24
6–12
2.4 × 107 Day 12
6–24
6–13
4.2 × 107 Days 12/13
Duodenum
6–24
6–9
6.8 × 103 Day 9
4–24
6–13
3.9 × 106 Days 12/13
Jejunum
6–17
6–12
1.1 × 107 Day 12
9–17
6–13
3.9 × 107 Days 12/13
Eyelid
6–24
9–17
9.7 × 107 Day 17
2–17
6–17
1.9 × 107 Days 12/13
Eye
6–24
9–17
1.2 × 107 Day 12
4–17
6–13
6.2 × 106 Days 12/13
Kidney
6–24
9–12
3.8 × 106 Day 12
6–17
9–13
4 × 106 Days 12/13
Gonad
6–24
9–17
7.6 × 107 Day 12
4–24
6–17
1 × 107 Days 12/13
Belly skin
6–24
9–17
3.3 × 105 Day 12
4–24
9–17
3.9 × 106 Days 12/13
Mes. lymph node
4–24
12
1.4 × 107 Day 12
6–17
9–13
1.2 × 106 Days 12/13
Stomach
4–24
12
4.3 × 105 Day 12
4–24
9–13
3.1 × 106 Days 12/13
Brain
6–24
12–17
7.1 × 104 Day 12
6–17
9–13
1.9 × 105 Days 12/13
Heart
4–24
12
1.4 × 106 Day 12
4–17
9–13
7.6 × 106 Days 12/13
Pancreas
6–17
12
2.8 × 106 Day 12
4–17
6–13
3.9 × 107 Days 12/13
Urine/bladder
6–24
12–17
3.5 × 106 Day 12
9–24
9–13
4.9 × 106 Days 12/13
Gallbladder
6–24
12–17
3.8 × 104 Day 12
4–24
12-13
2.2 × 106 Days 12/13
Feces
12–24
12–24
6.5 × 106 Day 12
12–24
12–17
1.1 × 107 Days 12/13
Lesion
12–24
12–24
7.8 × 107 Day 17
12–24
12–17
2.3 × 108 Days 12/13
Prairie dogs were intranasally challenged (8 × 103 p.f.u.) with West African (WA; ) or Congo Basin (CB; ) MPXV. Groups of prairie dogs were serially sacrificed (days 2, 4, 6, 9, 12, 17, and 24) following challenge. Postmortem samples were evaluated for presence of virus by real-time PCR and tissue culture. The time course of viral shedding for each tissue (viral DNA and viable virus) is shown above as is the peak viral load and day p.i. that the peak viral load was seen for each tissue. Peak viral loads were compared between MPXV clades; values for these comparisons were not statistically significant when comparing each tissue type (CB versus WA) and controlling for day of necropsy ( value range of 0.21–1).
