Simulated Microgravity: Critical Review on the Use of Random Positioning Machines for Mammalian Cell Culture

Figure 2

Mouse myoblasts (C2C12 cell line) were cultured until near confluence and subsequently exposed to a frequently passing air bubble. The culture chamber filled with medium was swinging upside down, such that the intentional air bubble frequently passed the same trajectory. The sample was fixed and stained for actin (green) and DNA (blue) thereafter. The cells in the trajectory of the air bubble got detached from the substrate (dark central area), while cells in the unaffected area kept proliferating (lateral green areas). Interestingly, detached cells could reattach to the opposite side of the culture chamber. Measuring bar 200 μm. (Due to the limited field of view, this image has been stitched together from five images.)