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BioMed Research International
Volume 2015, Article ID 972481, 8 pages
http://dx.doi.org/10.1155/2015/972481
Research Article

Pyrosequencing Reveals Fungal Communities in the Rhizosphere of Xinjiang Jujube

1College of Forest, Shandong Agricultural University, No. 61, Daizong Street, Taian, Shandong 271018, China
2College of Life Science, Shandong Agricultural University, Taian, Shandong 271018, China
3Research Institute of Forest in Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China

Received 18 September 2014; Revised 26 November 2014; Accepted 29 November 2014

Academic Editor: Ernesto Picardi

Copyright © 2015 Peng Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Fungi are important soil components as both decomposers and plant symbionts and play a major role in ecological and biogeochemical processes. However, little is known about the richness and structure of fungal communities. DNA sequencing technologies allow for the direct estimation of microbial community diversity, avoiding culture-based biases. We therefore used 454 pyrosequencing to investigate the fungal communities in the rhizosphere of Xinjiang jujube. We obtained no less than 40,488 internal transcribed spacer (ITS) rDNA reads, the number of each sample was 6943, 6647, 6584, 6550, 6860, and 6904, and we used bioinformatics and multivariate statistics to analyze the results. The index of diversity showed greater richness in the rhizosphere fungal community of a 3-year-old jujube than in that of an 8-year-old jujube. Most operational taxonomic units belonged to Ascomycota, and taxonomic analyses identified Hypocreales as the dominant fungal order. Our results demonstrated that the fungal orders are present in different proportions in different sampling areas. Redundancy analysis (RDA) revealed a significant correlation between soil properties and the abundance of fungal phyla. Our results indicated lower fungal diversity in the rhizosphere of Xinjiang jujube than that reported in other studies, and we hope our findings provide a reference for future research.