Targeted Mutation of Nuclear Bone Morphogenetic Protein 2 Impairs Secondary Immune Response in a Mouse Model
Calcium flux was not significantly reduced in compared to wild type lymph node or spleen leukocytes. Spleens (a and b) and lymph nodes (c and d) were harvested from wild type and mice on day 0 (naïve mice, a and c) and on day 38 (3 days after secondary infection, b and d). Calcium flux was measured using ionomycin stimulation of Fluo4-loaded cells in total leukocytes and in B220+ (B cells), CD4+ (helper T cells), and CD8+ (cytotoxic T cells) subgroups. Results shown are average ± SE ( per group, except for day 38 mutants, ).