Targeted Mutation of Nuclear Bone Morphogenetic Protein 2 Impairs Secondary Immune Response in a Mouse Model

Figure 8

Serum antibody titers were not different between wild type and mice following secondary infection. (a) Serum was harvested from wild type and mice on day 38 (3 days after secondary infection), and IgG antibody concentrations were measured by ELISA. Results shown represent the average ± SE ( for wild type, for mutant). (b) Antigen-specific antibody levels were determined using flow cytometry to measure the binding of serum IgG to intact S. aureus as described in Section 2.5. No statistically significant differences were seen in the levels of antigen-specific IgG from the serum of mutant compared to wild type mice. Results shown represent the average mean fluorescence intensity ± SE.

(a)

(b)