Research Article

Active Microbial Communities Inhabit Sulphate-Methane Interphase in Deep Bedrock Fracture Fluids in Olkiluoto, Finland

Table 1

The geochemical and biological measurements from the samples collected from fracture fluids from seven different boreholes in Olkiluoto, Finland. The different boreholes are presented as sampling depths.

296 m328 m347 m415 m559 m572 m798 m

BoreholeOL-KR13OL-KR6OL-KR23OL-KR49OL-KR2OL-KR1OL-KR29
Depth below ground surface (m)−296.11−328.37−346.52−415.45−559.15−572.24−797.81
Water typeBrackish SO4Brackish SO4SalineSalineSalineSalineSaline
Transmissivity (m2 s−1)5.86 × 10−81.31 × 10−76.48 × 10−74.37 × 10−74.33 × 10−75.50 × 10−7 (<10−9)
Hydrogeological zoneHZ001HZ20AHZ21HZ21
Pump rate (mL min−1)221042017223.962.16.1
Cumulative volume fracture fluid removed (L)11295486971750912514492496
Sampling date Microbiology9.3.201018.5.201015.12.200914.12.201027.1.201026.1.201018.5.2010
Sampling date Chemistry1.3.201010.5.20107.12.20091.12.200918.1.201018.1.20103.5.2010
Sampling date CH46.3.20063.8.200518.3.200313.5.20034.4.2005
Temperature (°C)19.611.617.61114.81217.7
pH7.97.97.58.18.67.87.3
Ec (mS m−1)897183221902670411037707820
DIC (mgC L−1)274.13.9<3<3.75<3.75<21
NPOC (mgC L−1)10<2.45.1<3115<12
TDS (mg L−1)4994106551273315899254592326153205
Alk (m) meq L−12.190.370.280.160.290.230.13
(mg L−1)79.53792.91.40.50.5<2
S2− (mg L−1)5.10NA0.620.02<0.020.13<0.02
NO3 (mg L−1)<0.01<0.01<0.01<0.01<0.01<0.01<0.01
NH4 (mg L−1)0.070.03<0.02<0.02<0.020.040.08
Fe2+ (mg L−1)<0.02NA0.080.53<0.020.400.46
Na2+ (mg L−1)1320280025303110498047209150
K+ (mg L−1)8.29.38.39.6192027
Ca2+ (mg L−1)4601100210027004600370010000
Mg2+ (mg L−1)357755191852136
Cl (mg L−1)2920623079309940157001460033500
CH4 (mL L−1)2222NANA386272920
TNC (mL−1)4.2 × 1051.0 × 1052.5 × 1051.5 × 1045.9 × 1048.7 × 1042.3 × 104
dsrB gene copies 3.1 × 104
(8.6 × 103)
5.4 × 103
(2.2 × 103)
1.4 × 104
(6.8 × 103)
1.6 × 104
(9.9 × 103)
6.5 × 101
(2.0 × 101)
2.2 × 103
(2.9 × 102)
0
dsrB transcripts 1.4 × 102
(1.5 × 102)
1.2 × 102
(7.0 × 101)
2.9 × 102
(1.8 × 102)
3.7 × 100
(1.6 × 100)
02.0 × 101
(9.0 × 100)
0
mcrA copies 7.5 × 100
(2.5 × 100)
05.4 × 101
(2.7 × 101)
04.6 × 102
(5.2 × 100)
2.5 × 101
(4.8 × 100)
0
mcrA transcripts 0000000

NA: data not available.
*Figure in brackets shows standard error of mean (SEM).