Exposure to Allergen Causes Changes in NTS Neural Activities after Intratracheal Capsaicin Application, in Endocannabinoid Levels and in the Glia Morphology of NTS

Figure 4

(a) Low magnification of NTS brain area and schematic representation (see Paxinos and Watson, 1986 [26]) of the area beside. (b, f, l) Iba-1 IR in NTS of a naive rat. (c, g, m) Iba-1 IR in NTS of sensitized rat to ovalbumin. (d, h, n) GFAP IR in NTS of a naive rat. (e, i, o) GFAP IR in NTS of sensitized rat. High magnification of Iba-1 + profiles (l, m) and GFAP + profiles (n, o) arrows. Scale bars = 100 μm. (A, B) Quantitative analysis of Iba1 and GFAP staining in NTS reveals significantly increased numbers of Iba-1 and GFAP-positive cells in the NTS after ovalbumin sensitization. Data represented as , rats per group. , compared to control group, one-way ANOVA, post hoc Tukey.