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BioMed Research International
Volume 2015, Article ID 983978, 6 pages
http://dx.doi.org/10.1155/2015/983978
Research Article

Comparative Evaluation of Different Test Combinations for Diagnosis of Mycobacterium avium Subspecies paratuberculosis Infecting Dairy Herds in India

1Animal Disease Research Centre, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, Punjab 141004, India
2Aquatic Animal Health Division, Central Institute of Brackishwater Aquaculture (CIBA), 75 Santhome High Road, R.A. Puram, Chennai 600028, India
3Microbiology Laboratory, Animal Health Division, Central Institute for Research on Goats, Makhdoom, P.O. Farah, Mathura, Uttar Pradesh 281122, India
4Department of Veterinary Medicine, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, Punjab 141004, India
5Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India
6National JALMA Institute for Leprosy and Other Mycobacterial Diseases (NJIL & OMD), Tajganj, Agra, Uttar Pradesh 282001, India
7Department of Microbiology, King George’s Medical University, Lucknow, Uttar Pradesh 226003, India
8Amity Institute of Microbial Technology, Amity University Rajasthan, Kant Kalwar, NH-11C Delhi-Jaipur Highway, Jaipur 303 002, India
9Department of Veterinary Microbiology and Immunology, Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu Chikitsa Vigyan Vishwa Vidyalaya Evam Go-Anusandhan Sansthan (DUVASU), Mathura, Uttar Pradesh 281001, India
10Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243122, India

Received 25 November 2014; Revised 24 February 2015; Accepted 9 March 2015

Academic Editor: Klaus Wimmers

Copyright © 2015 Rajni Garg et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A total of 355 cows were sampled (serum, ; faeces, ; milk, ) from dairy farms located in the Punjab state of India. Faeces and serum/milk samples were screened by acid fast staining and “indigenous ELISA,” respectively. IS900 PCR was used to screen faeces and milk samples. Bio-load of MAP in dairy cows was 36.9, 15.6, 16.3, and 14.4%, using microscopy, serum ELISA, milk ELISA and milk PCR, respectively. Estimated kappa values between different test combinations: serum and milk ELISA, faecal microscopy and faecal PCR, milk ELISA and milk PCR, faecal PCR and serum ELISA were 0.325, 0.241, 0.682, and 0.677, respectively. Estimation of the relative sensitivity and specificity of different tests in the present study indicated that “serum ELISA” and “milk ELISA” were good screening tests, add “milk PCR” was “confirmatory test” for MAP infection. Combination of milk ELISA with milk PCR may be adopted as a model strategy for screening and diagnosis of JD in lactating/dairy cattle herds in Indian conditions.