Dietary Flavonoid Hyperoside Induces Apoptosis of Activated Human LX-2 Hepatic Stellate Cell by Suppressing Canonical NF-κB Signaling

Table 1

Primers of quantitative real-time PCR.

Primers	Sequence		Annealing temperature (°C)
	Forward (from 5′ to 3′)	Reverse (from 5′ to 3′)
18s	CAGCCACCCGAGATTGAGCA	TAGTAGCGACGGGCGGTGTG	60
-SMA	AGGTAACGAGTCAGAGCTTTGGC	CTCTCTGTCCACCTTCCAGCAG	60
Collagen I	GAGCGGAGAGTACTGGATCG	GTTCGGGCTGATGTACCAGT	60
A20	GTCCGGAAGCTTGTGGCGCT	CCAAGTCTGTGTCCTGAACGCCC	55
Bcl-Xs	GCAGTAAAGCAAGCGCTGAG	GTTCCACAAAAGTATCCTGTTCAAAG	60
DR4	CTGAGCAACGCAGACGCGCTGTCCAC	ACAGCATCAGAGTCTCAGTGGGGTCAGC	60
Fas	ATTCTGCCATAAGCCCTGTC	TTGGTGTTGCTGGTGAGTGT	55
FasL	GTTCTGGTTGCCTTGGTAGG	TGTGCATCTGGCTGGTAGAC	55
c-IAP1	TTGTCAACTTCAGATACCACTGGAG	CAAGGCAGATTTAACCACAGGTG	60
Bcl-X	AGTTCCCTTGGCCTCAGAAT	AGGGTTGCACCAATCAGGTA	55
Rip1	GTCAAATTCAGCCACAGAACAGCC	CCCTTTAGCCTTCCCTCATCACC	55