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BioMed Research International
Volume 2016, Article ID 1253842, 6 pages
http://dx.doi.org/10.1155/2016/1253842
Research Article

Diazoxide Attenuates Postresuscitation Brain Injury in a Rat Model of Asphyxial Cardiac Arrest by Opening Mitochondrial ATP-Sensitive Potassium Channels

1Department of Emergency Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China
2Institute of Cardiopulmonary Cerebral Resuscitation, Sun Yat-sen University, Guangzhou, China

Received 9 March 2016; Accepted 3 August 2016

Academic Editor: Konstantinos Pappas

Copyright © 2016 Haidong Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1. Comparison of cerebral cell mitochondrial respiratory parameters in different groups 24 h after ROSC. Data are presented as means ± SD, n = 5 rats/group. *P < 0.05 vs. sham group, #P < 0.05 vs. vehicle group, **P < 0.05 vs. DZ group. 5-HD: 5-hydroxydecanoate; DZ: diazoxide; RCR: respiratory control rate.

Supplementary Figure 2. TUNEL staining analysis following ROSC

A: Quantitative analysis of TUNEL-positive cells in cortical regions 24 h after ROSC in the four rat groups. Five randomly-selected high-power (× 400) fields were analyzed per section, and the percentage of positive cells (positive cells/total cells × 100%) was calculated to determine the apoptotic index. Data are presented as means ± SD, n = 5 rats/group. *P < 0.01 vs. sham group; #P < 0.05 vs. vehicle group; **P < 0.05 vs. DZ group

B: Representative photomicrographs of TUNEL staining. 5-HD: 5-hydroxydecanoate; DZ: diazoxide. ROSC: return of spontaneous circulation

Supplementary Figure 3. Expression of Bcl-2, Bax, and PKCε protein following ROSC. Expression of Bcl-2, Bax, and PKCε protein in the cerebral cortex 24 h after ROSC in a rat model of cardiac arrest was assessed using Western blotting (A). Western blots were quantified by densitometry (BE). Data are presented as means ± SD, n = 5 rats/group. *P < 0.01 vs. sham group; #P < 0.05 vs. vehicle group; **P < 0.05 vs. DZ group; ROSC: return of spontaneous circulation; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.

Supplementary Figure 4. Expression of Bcl-2, Bax, and PKCε protein in the cerebral cortex 24 h after ROSC in the four rat groups obtained via immunohistochemical staining. (A, E, I) Sham; (B, F, J) Vehicle; (C, G, K) DZ; (D, H, L) DZ+5-HD; ROSC: return of spontaneous circulation.

  1. Supplementary Material