In Vitro and In Vivo Biofilm Characterization of Methicillin-Resistant Staphylococcus aureus from Patients Associated with Pharyngitis Infection

Figure 3

Total biofilm formation of different clinical MRSA isolates. (a) The bacterial cells were grown in 24-well Mtps containing TSB supplemented with 0.25% glucose. The cells that adhered to the plate surface after washing with phosphate buffer were visualized by crystal violet staining. The isolates were considered as highly adherent (a1), strongly adherent (a2), moderately adherent (a3), and nonadherent (a4) based upon their absorbance at 570 nm as measured by spectrophotometer. (b) Confocal laser scanning micrographs revealing variable degrees of biofilm production by clinical MRSA isolates on glass surface: (b1) highly adherent; (b2) strongly adherent; (b3) moderately adherent; and (b4) nonadherent isolates. (c) COMSTAT analysis of the obtained CLSM images.

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