In Vitro and In Vivo Biofilm Characterization of Methicillin-Resistant Staphylococcus aureus from Patients Associated with Pharyngitis Infection
Table 2
Relationships among the presence of icaA, icaD, icaB, icaC, fnbA, fnbB, clfA, cna, hla, and sarA genes, slime production, adherence capacity on MtPs, and hydrophobicity index in 63 different clinical MRSA associated with GAS pharyngitis infection.
Strain ID
Biofilm phenotype on CRA
Slime synthesis
In vitro adherence (MtP) assay
Hydrophobicity index ± SD
Presence of adhesion genes
Adherence OD570 nm ± SD
Adherence ability
icaA
icaB
icaC
icaD
sarA
cna
clfA
fnbA
fnbB
hla
GSA-83
Black
Producer
1.42 ± 0.213
++
26.3 ± 0.325
+
+
+
+
+
+
+
+
+
+
GSA-22
Black
Producer
1.79 ± 0.659
++
32.3 ± 0.336
+
+
+
+
+
+
+
+
+
+
GSA-32
Strong black
Producer
3.23 ± 0.986
+++
29.4 ± 0.962
+
+
+
+
+
+
+
+
+
+
GSA-A21
Black
Producer
1.53 ± 0.286
++
40.3 ± 0.560
+
+
+
+
+
+
−
+
−
−
GSA-127
Black
Producer
0.91 ± 0.632
+
32.9 ± 0.123
−
−
−
−
+
−
−
−
+
+
GSA-74
Strong black
Producer
3.21 ± 0.215
+++
41.3 ± 0.963
+
+
+
+
+
+
−
−
−
−
GSA-45
Strong black
Producer
3.09 ± 0.0236
+++
44.2 ± 0.023
+
+
+
+
+
+
+
+
+
+
GSA-150
Reddish black
Nonproducer
0.19 ± 1.023
15.3 ± 0.965
−
−
−
−
+
−
+
+
+
+
GSA-99
Reddish black
Nonproducer
1.32 ± 0.963
++
16.4 ± 0.189
+
+
+
+
−
+
−
−
+
+
GSA-103
Bordeaux red
Nonproducer
0.47 ± 0.451
21.6 ± 0.651
+
+
+
+
+
+
+
+
+
+
GSA-89
Strong black
Producer
3.41 ± 0.238
+++
29.3 ± 0.359
+
+
+
+
+
+
+
+
+
+
GSA-50
Black
Producer
3.06 ± 0.896
+++
31.2 ± 0.158
+
+
+
+
+
+
+
+
+
+
GSA-A8
Strong black
Producer
2.53 ± 1.639
++
27.9 ± 0.958
+
+
+
+
+
−
+
+
+
+
GSA-44
Strong black
Producer
3.86 ± 0.127
+++
24.6 ± 0.756
+
+
+
+
+
+
+
−
−
−
GSA-46
Bordeaux red
Nonproducer
0.39 ± 0.986
24.3 ± 0.286
+
+
+
+
+
+
−
+
+
+
GSA-48
Black
Producer
1.06 ± 1.028
+
20.9 ± 0.396
+
+
+
+
+
+
−
+
+
+
GSA-54
Bordeaux red
Nonproducer
0.49 ± 0.966
14.5 ± 0.362
−
−
−
−
+
−
+
−
+
+
GSA-395
Strong black
Producer
3.23 ± 0.523
+++
29.5 ± 0.396
+
+
+
+
+
+
+
+
+
+
GSA-68
Strong black
Producer
3.51 ± 0.889
+++
32.6 ± 0.325
+
+
+
+
+
+
+
+
+
+
GSA-94
Bordeaux red
Nonproducer
0.42 ± 0.365
18.6 ± 0.176
−
−
−
−
−
−
−
−
−
−
GSA-104
Black
Producer
1.48 ± 0.632
++
30.9 ± 0.963
−
−
−
−
−
+
+
−
+
+
GSA-140
Strong black
Producer
3.52 ± 0.023
+++
36.2 ± 0.990
+
+
+
+
+
+
−
+
−
−
GSA-145
Strong black
Producer
3.22 ± 0.965
+++
28.9 ± 1.230
+
+
+
+
+
+
−
+
−
−
GSA-142
Reddish black
Nonproducer
0.96 ± 1.036
+
30.6 ± 0.968
−
−
−
−
+
−
−
−
−
−
GSA-70
Bordeaux red
Nonproducer
0.23 ± 0.396
13.6 ± 0.869
−
−
−
−
−
−
−
+
+
+
GSA-126
Strong black
Producer
3.62 ± 0.325
+++
43.6 ± 0.310
+
+
+
+
+
+
+
+
+
+
GSA-A4
Black
Producer
2.57 ± 0.635
++
21.5 ± 0.256
+
+
+
+
+
+
+
+
+
+
GSA-92
Reddish black
Nonproducer
0.39 ± 0.961
12.9 ± 0.178
+
+
+
+
+
−
−
+
+
+
GSA-365
Black
Producer
1.98 ± 0.362
++
36.5 ± 0.986
+
+
+
+
+
−
+
+
+
−
GSA-A12
Black
Producer
1.59 ± 0.589
++
21.9 ± 0.936
+
+
+
+
+
+
+
+
+
+
GSA-A18
Bordeaux red
Nonproducer
1.09 ± 0.698
+
19.2 ± 0.129
+
+
+
+
+
−
−
+
+
−
GSA-297
Black
Producer
1.96 ± 0.129
++
29.9 ± 0.326
+
+
+
+
+
+
+
+
+
+
GSA-134
Reddish black
Nonproducer
3.12 ± 0.396
+++
13.6 ± 0.349
+
+
+
+
+
+
−
+
+
−
GSA-75
Black
Producer
1.79 ± 0.326
++
30.1 ± 0.559
+
+
+
+
+
+
+
+
+
+
GSA-88
Strong black
Nonproducer
3.09 ± 0.856
+++
24.3 ± 0.552
+
+
+
+
+
+
+
+
+
+
GSA-71
Black
Producer
3.85 ± 0.785
+++
22.3 ± 0.639
+
+
+
+
+
+
+
+
+
+
GSA-A25
Black
Producer
0.85 ± 0.759
+
26.8 ± 1.36
+
+
+
+
+
−
+
+
+
+
GSA-79
Bordeaux red
Nonproducer
0.21 ± 0.856
−
14.9 ± 0.759
−
−
−
−
−
+
−
+
+
−
GSA-52
Black
Producer
3.52 ± 0.996
+++
23.6 ± 0.529
+
+
+
+
+
+
+
−
−
+
GSA-91
Bordeaux red
Nonproducer
0.86 ± 1.236
+
15.9 ± 0.169
+
+
+
+
+
−
+
+
+
+
GSA-84
Strong black
Producer
3.11 ± 1.036
+++
40.1 ± 0.629
+
+
+
+
+
+
−
+
+
+
GSA-53
Bordeaux red
Nonproducer
0.36 ± 0.845
−
19.1 ± 0.785
+
+
+
+
+
−
+
+
+
+
GSA-137
Black
Producer
1.56 ± 0.965
++
22.6 ± 0.396
+
+
+
+
+
+
−
+
+
+
GSA-A20
Strong black
Producer
3.51 ± 0.515
+++
42 ± 0.968
+
+
+
+
+
+
−
+
+
+
GSA-291
Black
Producer
1.63 ± 0.689
++
26.9 ± 0.236
+
+
+
+
+
+
−
+
+
+
GSA-98
Black
Producer
1.79 ± 0.632
++
22.6 ± 0.756
+
+
+
+
+
+
−
+
+
+
GSA-73
Strong black
Producer
3.24 ± 0.325
+++
36.2 ± 0.688
+
+
+
+
+
+
−
+
+
+
GSA-A16
Black
Producer
2.06 ± 0.963
++
32.8 ± 0.895
+
+
+
+
−
+
+
+
+
+
GSA-131
Bordeaux red
Nonproducer
1.08 ± 0.896
+
16.3 ± 0.955
+
+
+
+
+
−
−
+
+
−
GSA-410
Strong black
Producer
3.69 ± 0.563
+++
36.8 ± 0.269
+
+
+
+
+
+
+
+
+
+
GSA-377
Bordeaux red
Nonproducer
0.27 ± 1.342
−
17.2 ± 0.745
+
+
+
+
+
+
+
+
+
−
GSA-A1
Black
Producer
3.04 ± 0.506
+++
21.1 ± 0.986
+
+
+
+
+
+
+
−
−
+
GSA-A6
Bordeaux red
Nonproducer
0.79 ± 0.966
+
13.9 ± 0.156
+
+
+
+
+
+
+
+
+
−
GSA-A9
Strong black
Nonproducer
2.89 ± 0.796
++
24.3 ± 0.969
+
+
+
+
+
+
+
+
+
+
GSA-A17
Black
Producer
1.85 ± 0.235
+++
19.9 ± 0.589
+
+
+
+
+
+
+
+
+
+
GSA-28
Bordeaux red
Nonproducer
1.25 ± 0.168
+
19.2 ± 0.129
+
+
+
+
+
+
+
−
+
−
GSA-51
Black
Producer
2.57 ± 0.234
++
21.3 ± 0.345
+
+
+
+
+
+
−
+
+
+
GSA-58
Reddish black
Nonproducer
0.55 ± 0.996
+
18.2 ± 0.569
+
+
+
+
+
+
+
+
+
−
GSA-63
Reddish black
Nonproducer
1.43 ± 0.351
++
11.9 ± 0.266
−
−
−
−
−
−
−
−
−
−
GSA-81
Reddish black
Nonproducer
0.98 ± 0.029
+
19.1 ± 0.192
+
+
+
+
−
−
−
−
−
+
GSA-86
Bordeaux red
Nonproducer
0.49 ± 0.259
−
16.2 ± 0.367
−
−
−
−
−
−
+
−
−
−
GSA-97
Strong black
Nonproducer
3.39 ± 0.125
+++
23.9 ± 0.121
+
+
+
+
+
+
+
+
+
+
GSA-102
Black
Producer
1.08 ± 0.985
+
24.5 ± 0.276
+
+
+
+
+
+
−
+
+
−
: Indicating the varied adhering ability of isolates on polystyrene surface, where +++ represents highly adherent (OD570 values of >3.0), ++ represents strongly adherent (OD570 values of >2.0), + represents moderately adherent (OD570 values of >1.0–2.0) and − represents weakly adherent (OD570 values of >0.5–1.0).
