Exposure to p,p′-DDE Induces Morphological Changes and Activation of the PKCα-p38-C/EBPβ Pathway in Human Promyelocytic HL-60 Cells

Figure 5

The p,p′-DDE exposure increases . HL-60 cells loaded with Fluo-3-AM in the absence of extracellular Ca²⁺ were used to determine by flow cytometry. (a) The data shown correspond to a representative experiment measuring fluorescence where the baseline was registered for 20 s, followed by the addition of p,p′-DDE (as indicated by the arrow) and subsequent monitoring for 200 s. (b) Bars correspond to the maximum increase in after addition of p,p′-DDE. (c) Fluorescence collection corresponding to in cells exposed to 1.9 μg/mL of p,p′-DDE with or without BAPTA-AM in the absence of extracellular [Ca²⁺]; results are from a representative experiment. (d) Bars correspond to the maximum increase in . Data from three independent experiments are expressed as the mean ± SEM. indicates differences relative to baseline levels. indicates differences relative to the sample exposed to 1.9 μg/mL of p,p′-DDE. Data were analysed using a one-way ANOVA followed by Dunnett’s post hoc test.

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