(a)
(b)
(c)
(d)
Figure 5: The p,p-DDE exposure increases . HL-60 cells loaded with Fluo-3-AM in the absence of extracellular Ca2+ were used to determine by flow cytometry. (a) The data shown correspond to a representative experiment measuring fluorescence where the baseline was registered for 20 s, followed by the addition of p,p-DDE (as indicated by the arrow) and subsequent monitoring for 200 s. (b) Bars correspond to the maximum increase in after addition of p,p-DDE. (c) Fluorescence collection corresponding to in cells exposed to 1.9 μg/mL of p,p-DDE with or without BAPTA-AM in the absence of extracellular [Ca2+]; results are from a representative experiment. (d) Bars correspond to the maximum increase in . Data from three independent experiments are expressed as the mean ± SEM. indicates differences relative to baseline levels. indicates differences relative to the sample exposed to 1.9 μg/mL of p,p-DDE. Data were analysed using a one-way ANOVA followed by Dunnett’s post hoc test.