(a) T + DIOL
(b) DIOL
(c) T
(d) DIOL/T
Figure 2: Concentration dependent effects of DBP on basal androgen production by rat immature Leydig cells. Rat immature Leydig cells were cultured with 0.05–50 μM dibutyl phthalate (DBP) for 3 hrs. Medium testosterone (T) and 5α-androstanediol (DIOL) levels were measured. (a) T + DIOL; (b) DIOL; (c) T; and (d) DIOL/T ratio. Mean ± SEM, ; , , and indicate significant difference when compared to control at , 0.01, and 0.001, respectively.