Comparison of the Effects of Dibutyl and Monobutyl Phthalates on the Steroidogenesis of Rat Immature Leydig Cells

Figure 4

Effects of DBP on androgen production by rat immature Leydig cells. Rat immature Leydig cells were cultured without or with luteinizing hormone (LH), 8bromo-cAMP (8Br-cAMP), 22R-OH-cholesterol (22R-OHC), pregnenolone (P5), progesterone (P4), and androstenedione (D4) in combination with 50 μM DBP for 3 hrs. Medium 5α-androstanediol (DIOL) and testosterone (T) levels were measured. Mean ± SEM, ; , , and indicate significant difference when compared to control at , 0.01, and 0.001, respectively.

(a) Basal

(b) LH

(c) 8Br-cAMP

(d) 22R-OHC

(e) P5

(f) P4

(g) D4