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BioMed Research International
Volume 2016, Article ID 1393089, 9 pages
Research Article

Methylation Status of Alu and LINE-1 Interspersed Repetitive Sequences in Behcet’s Disease Patients

1Center of Excellence in Immunology and Immune-Mediated Diseases, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
2Ankara Education and Research Hospital, Altindag, 06340 Ankara, Turkey
3Gazi University Faculty of Medicine, Yenimahalle, 06560 Ankara, Turkey

Received 6 January 2016; Revised 17 February 2016; Accepted 6 March 2016

Academic Editor: Yves Renaudineau

Copyright © 2016 Şahru Yüksel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Behcet’s Disease (BD) is a multisystem chronic inflammatory disease. The pathology is believed to involve both genetic susceptibility and environmental factors. Hypomethylation leading to activation of interspersed repetitive sequences (IRSs) such as LINE-1 and Alu contributes to the pathologies of autoimmune diseases and cancer. Herein, the epigenetic changes of IRSs in BD were evaluated using combined bisulfite restriction analysis-interspersed repetitive sequences (COBRA-IRS). DNA from neutrophils and peripheral blood mononuclear cells (PBMCs) of BD patients with ocular involvement that were in active or inactive states and healthy controls were used to analyze LINE-1 and Alu methylation levels. For Alu sequences, significant differences were observed in the frequency of alleles between PBMCs of patients and controls (), and between inactive patients and controls (). For neutrophils, the frequency of was significantly higher between patients and controls () and between inactive patients and controls (). The partial methylation () frequencies of Alu between inactive patients and control samples also differed (). No statistically significant differences for LINE-1 were detected. Thus, changes in the methylation level of IRS elements might contribute to the pathogenesis of BD. The role of Alu transcripts in BD should be investigated further.