Manipulation of DNA Repair Proficiency in Mouse Models of Colorectal Cancer

Table 1

Genetically engineered alleles of the mouse Apc gene.

Apc allele	Mutation	Polyp number	Pathology/comments	Ref
	Frameshift at codon 850; (ENU induced)	~30–>100	Polypoid, papillary and sessile adenomas; cystic crypts, no colonic ACF	[26]
	Floxed exons 1–15,; no protein	~210 ♀; ~150 ♂	Polypoid, papillary and sessile adenomas; cystic crypts, no colonic ACF	[27]
	Floxed exon 15, frameshift at codon 650, truncation at 667	~185	Polypoid and sessile adenomas; cystic crypts; few colonic lesions	[28]
Apc^1322T/+	Neomycin cassette inserted into exon 15; stop at codon 1322	~200	Polyps predominantly in the first and second segments of the small intestine; few gastric and colonic polyps; polyps have reduced Wnt signaling relative to Apc^Min polyps	[29]
	Floxed exon 14, frameshift at codon 580, truncation at 605	~7	Exposure to adenoviral-Cre; adenomas localized near anus	[30]
	Floxed exon 14, frameshift at codon 580, truncation at 605	~65	Polypoid and sessile adenomas; increase in colonic polyps, ACF, and rectal prolapses	[31]
	Floxed exon 14, frameshift at codon 580, truncation at 605	~120	Crossed to K14-Cre mouse line; polyploid and sessile adenomas; increase in colonic polyps, with additional abnormalities in the skin, thymus, and tooth	[32]
Apc^1638N	Neomycin cassette inserted into exon 15 in antisense; frameshift at codon 1638	~10	Colonic polyploid hyperplastic lesions, villous/tubulovillous adenomas; moderately to highly differentiated adenocarcinoma; rare gastric lesions	[33]
	PGK-hygromycin cassette inserted in sense orientation; stop at codon 1638	0	Developmental abnormalities; growth retardation; absence of preputial glands	[34]
	Neomycin cassette inserted into exon 15; frameshift at codon 716	~254	Polypoid, papillary, and sessile adenomas; no colonic ACF	[35]
	Frameshift at codon 1309	~34	Polyps mainly in small intestine but also in the stomach and colon	[36]
	Neomycin cassette inserted into exon 9; duplication of exons 7–10; frameshift at codon 474	~122	Sessile-type polyps; rare mammary adenocarcinomas	[37]
Apc^neoF; Apc^neoR	Neomycin cassette inserted into intron 13 in both sense and antisense orientations	neoF ~ 1.00; neoR ~ 0.25	Dysplastic adenomas similar to those from mice	[38]

A more extensive list of Apc mouse alleles can be found at http://www.informatics.jax.org/marker/phenotypes/MGI:88039.