Metformin Alleviated Aβ-Induced Apoptosis via the Suppression of JNK MAPK Signaling Pathway in Cultured Hippocampal Neurons

Figure 4

Metformin decreased Aβ-induced cytotoxicity by suppression of apoptosis. Representative results of JC-10 staining. Quantitative analysis of fluorescence intensity showed that Aβ increased the phosphorylation level of JNK, which was reserved by metformin (, ). However, when β-lapachone, the activator of apoptosis, was added into the medium, the metformin-involved protection was blocked, indicating metformin played its role via decreasing apoptosis; scale bars, 50 μm.