Inhibitor of Nicotinamide Phosphoribosyltransferase Sensitizes Glioblastoma Cells to Temozolomide via Activating ROS/JNK Signaling Pathway

Figure 2

NAMPT inhibitor increases the TMZ-induced apoptosis and necrosis in glioblastoma cells. (a) Fluorescent images of TUNEL assay and quantitative analysis showing the effect of low doses of FK866 and CHS828 (10 nM and 20 nM, resp.) on the apoptosis in U251-MG GBM cells. DAPI was used to stain nuclei. versus TMZ alone. . At least 20 visual fields were included for analysis. (b-c) LDH assay showing the LDH content in culture medium of TMZ alone, TMZ plus FK866, and TMZ plus CHS828 treated U251-MG GBM cells. and versus TMZ alone. .

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