Phosphorylated and Nonphosphorylated PfMAP2 Are Localized in the Nucleus, Dependent on the Stage of Plasmodium falciparum Asexual Maturation

Figure 1

Expression levels of PfMAP2 at different stages of P. falciparum. (a) Expression pattern of PfMAP2 and phosphorylated PfMAP2 (PPfMAP2) within the developmental stages of P. falciparum. Stage-specific Western blot analyses were performed. H, host; R, ring stage; T, trophozoite stage; S, schizont stage. β-actin was used as a standard to allow semiquantitative comparison between samples over time. (b) Result shown is the average value of triplicate result () +SE. A repeated measures 2-way ANOVA with Bonferroni post hoc analysis showed that the overall RI of (PfMAP and PPfMAP2) significantly decreased as the parasite developed from ring to schizont (, DF = 2,12 ), and that the RI of PPfMAP2 was always significantly higher than PfMAP2. However the ratio of PfMAP2 and PPfMAP2 did not significantly differ between stages of development; that is, there was no interaction effect (, DF = 2,12 (not shown in the figure)). Immunoblotting was carried out using PfMAP2 peptide and PPfMAP2 peptide (1 : 3000).

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