Phosphorylated and Nonphosphorylated PfMAP2 Are Localized in the Nucleus, Dependent on the Stage of Plasmodium falciparum Asexual Maturation

Figure 2

Subcellular distribution of PfMAP2 in P. falciparum. Immunofluorescence analysis of (a) PfMAP2 without phosphatase treatment localization in P. falciparum-infected red blood cells, (b) PfMAP2 with phosphatase treatment localization in P. falciparum-infected red blood cells, (c) PPfMAP2 without phosphatase treatment localization in P. falciparum-infected red blood cells, and (d) PPfMAP2 with phosphatase treatment localization in P. falciparum-infected red blood cells. P. falciparum-infected red blood cells were treated with DAPI (blue) and incubated with primary antibody PfMAP2 (1 : 100) and secondary antibody conjugated-FITC (green) (1 : 200).

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