Tumor Take Rate Optimization for Colorectal Carcinoma Patient-Derived Xenograft Models
Table 2
Tumor ID
Outcome
Days frozen
No cooling
24 h cooling
HROC107
M1(+/+); M2(+/−)
M3(−/+); M4(−/+)
293
HROC118
M1(−/+); M2(−/−)
M3(−/+); M4(−/+)
174
HROC119
M1(−/−); M2(−/−)
M3(−/−); M4(−/−)
154
HROC122
M1(−/−); M2(−/−)
M3(−/−); M4(−/−)
130
HROC123
M1(+/−); M2(−/−)
M3(−/−); M4(−/−)
130
HROC125
M1(−/−); M2(−/−)
M3(−/−); M4(−/−)
74
HROC129
M1(+/+); M2(−/+)
M3(−/−); M4(−/−)
61
HROC130
M1(−/+); M2(−/−)
M3(−/−); M4(−/−)
57
HROC131
M1(+/+); M2(+/+)
M3(+/+); M4(+/+)
54
HROC135
M1(−/+); M2(−/−)
M3(−/−); M4(−/+)
40
Ø Matrigel
6/20
2/20
With Matrigel
8/20
7/20
A total of 4 mice were implanted on both flanks with human CRC tissue samples. M denotes individual animals xenografted; the outcome is given in parentheses as index + (outgrowing tumor) or index − (no outgrowth). Tumor tissues presoaked with Matrigel are indicated in underlined signs. The overall number of successful sites with and without the addition of Matrigel is given for the total of 20 sides implanted.
