Review Article

Plant Responses to High Frequency Electromagnetic Fields

Table 1

Metabolic pathways affected after plant exposure to HF-EMF radiations.

Enzymes or metabolitesMetabolic pathwaysOrganismsExposure conditionsResponse to EMF

Phenylalanine ammonia-lyasePhenylpropanoidsPhaseolus vulgarisN/A (PEMF)Synergistic action with growth regulators in cultured cells [26]

Polyphenol oxidasePolyphenolsVigna radiata900 MHz, up to 4 h, 8.55 μW cm−28.5-fold increase [27]

α- and β-amylasesStarch metabolismVigna radiata900 MHz, up to 4 h, 8.55 μW cm−22.5- and 15-fold increase for α- and β-amylases, respectively [27]

α- and β-amylases, starch phosphorylasesStarch metabolismZea mays1800 MHz, up to 4 h, 332 mW m−22-fold increase for amylases. −73% for starch phosphorylases [28]

Water soluble sugarsSugar metabolismPhaseolus vulgaris900 MHz, 4 h2-fold reduction in soluble sugars [12]

Acid and alkaline invertasesSucrose metabolismZea mays1800 MHz, up to 4 h, 332 mW m−21.8- and 2.6-fold increase for acid and alkaline forms, respectively [28]

Malate and NADP isocitrate dehydrogenases, glucose-6P dehydrogenase Krebs cycle, pentose phosphate pathwayPlectranthus900 MHz, 1 hLower activity (−10 to −30%) at the end of the stimulus and then a 2-fold increase 24 h later [29]

ATP content and adenylate energy charge (AEC)Energetic metabolismSolanum lycopersicon900 MHz, 10 min, 5 V m−1Drop of ATP content (30%) and AEC (0.8 to 0.6) 30 min after the stimulus [10]

MDA content, H2O2, superoxide dismutase, catalase, guaiacol peroxidase, glutathione reductase, ascorbate peroxidaseLipid peroxidation-oxidative metabolismVigna radiata900 MHz, 8.55 μW cm−2All oxidative metabolism markers increased (2-fold to 5-fold) [7]

MDA and H2O2 content, catalase, ascorbate peroxidaseLipid peroxidationLemna minor400 and 900 MHz, 2 to 4 h, 10 to 120 V m−1MDA and H2O2 content, catalase and ascorbate peroxidase activities increased (10–30%) [30]

PeroxidasesOxidative metabolismVigna radiata, Lemna minor900 MHz, 1 to 4 h, 8.55 μW cm−2 or 41 V m−1Peroxidase activities increased [18, 27]

MDA, oxidized and reduced glutathione, NO synthaseOxidative metabolism-NO metabolismTriticum aestivum2.45 GHz, 5 to 25 s, 126 mW mm−2 concomitantly with NaCl treatmentExposure to EMF reduced the oxidative response of plants to high salt treatment [31]

Protein metabolism-DNA damageOxidative protein and DNA damage (comet assay)Nicotiana tabacum900 MHz, 23 V m−1Carbonyl content and tail DNA value increased (1.8-fold and 30%, resp.) [30]

Protein metabolismProtein contentPhaseolus vulgaris, Vigna radiata, Triticum aestivumCell phone, 4 hDrop in protein content in Phaseolus (71%) and Vigna (57%) [27, 32] and Triticum [13]

Amino acid metabolismProline accumulationZea mays, Vigna radiata940 MHz, 2 days
Cell phone, 2 h, 8.55 μW cm−2
1.8- and 5-fold increase in Z. mays [33] and V. radiata [7], respectively

Global terpene emissionMonoterpene metabolismPetroselinum crispum, Apium graveolens, Anethum graveolens900–2400 MHz,
70–100 mW m−2
Enhanced emission of terpene compounds [34]