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BioMed Research International
Volume 2016, Article ID 1859254, 14 pages
http://dx.doi.org/10.1155/2016/1859254
Research Article

Effects of Shaoyao-Gancao Decoction on Infarcted Cerebral Cortical Neurons: Suppression of the Inflammatory Response following Cerebral Ischemia-Reperfusion in a Rat Model

1Department of Rehabilitation, Shanghai Xuhui Central Hospital, Shanghai Clinical Center, Chinese Academy of Sciences, No. 966, Huaihai Road, Shanghai 200031, China
2School of Life Sciences, Shanghai University, No. 99 Shangda Road, Shanghai 200444, China
3School of Medicine, Jiao Tong University, No. 280, Chongqing South Road, Shanghai 200025, China
4Department of Chinese Medicine, Shanghai Punan Hospital, No. 519, Pudong New Area South Wharf Road, Shanghai 200125, China
5Department of Rehabilitation, Shanghai Dahua Hospital, No. 903, Old Humin Road, Xuhui District, Shanghai 200237, China

Received 9 March 2016; Revised 26 May 2016; Accepted 26 May 2016

Academic Editor: Fabrizio Montecucco

Copyright © 2016 Ying Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The mechanisms by which Shaoyao-Gancao decoction (SGD) inhibits the production of inflammatory cytokines in serum and brain tissue after cerebral ischemia-reperfusion (CI-RP) in rats were investigated. A right middle cerebral artery occlusion was used to induce CI-RP after which the rats were divided into model (), SGD (), clopidogrel () and sham operated () groups. The Bederson scale was used to evaluate changes in behavioral indices. The levels of IL-1β, TNF-α, MCP-1, IL-10, RANTES, VEGF, and TGF-β1 in the serum and infarcted brain tissues were measured. Nissl body and immunohistochemical staining methods were used to detect biochemical changes in neurons, microglial cells, and astrocytes. Serum levels of VEGF, TNF-α, MCP-1, IL-1β, and IL-10 increased significantly 24 h after CI-RP. In brain tissue, levels of TNF-α and IL-1β significantly increased 24 h after CI-RP, whereas levels of TGF-β1 and MCP-1 were significantly higher 96 h after CI-RP (). SGD or clopidogrel after CI-RP reduced TNF-α and IL-1β levels in brain tissue and serum levels of MCP-1, IL-1β, and IL-10. SGD increased the number of NeuN-positive cells in infarcted brain tissue and reduced the number of IBA1-positive and GFAP-positive cells. The efficacy of SGD was significantly higher than that of clopidogrel.