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BioMed Research International
Volume 2016, Article ID 1974972, 4 pages
Research Article

Different Vancomycin Immunoassays Contribute to the Variability in Vancomycin Trough Measurements in Neonates

1Institute of Pharmacology, Clinical Pharmacology and Toxicology, Medical Faculty, University of Belgrade, 11000 Belgrade, Serbia
2Division of Pediatric Pharmacology and Pharmacometrics, University of Basel Children’s Hospital, 4056 Basel, Switzerland
3Department of Development and Regeneration, KU Leuven, 3000 Leuven, Belgium
4Neonatal Intensive Care Unit, University Hospitals Leuven, 3000 Leuven, Belgium
5Clinical Pharmacology and Pharmacotherapy, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven and Pharmacy Department, University Hospitals Leuven, 3000 Leuven, Belgium
6Medical Faculty, University of Belgrade, 11000 Belgrade, Serbia
7Clinical Pharmacology Unit, University Children’s Hospital, 11000 Belgrade, Serbia
8Division of Pediatric Clinical Pharmacology, Children’s National Medical Center, Washington, DC 20010, USA
9Intensive Care and Department of Pediatric Surgery, Erasmus MC Sophia Children’s Hospital, 3000 CB Rotterdam, Netherlands

Received 18 February 2016; Accepted 31 July 2016

Academic Editor: Giulio Mengozzi

Copyright © 2016 Janko Samardzic et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Substantial interassay variability (up to 20%) has been described for vancomycin immunoassays in adults, but the impact of neonatal matrix is difficult to quantify because of blood volume constraints in neonates. However, we provide circumstantial evidence for a similar extent of variability. Using the same vancomycin dosing regimens and confirming similarity in clinical characteristics, vancomycin trough concentrations measured by PETINIA (2011-2012, ) were 20% lower and the mean difference was 1.93 mg/L compared to COBAS (2012–2014, ) measurements. The impact of vancomycin immunoassays in neonatal matrix was hereby suggested, supporting a switch to more advanced techniques (LC-MS/MS).