Wnt-β-Catenin Signaling Promotes the Maturation of Mast Cells

Figure 5

Effect of Wnt5a inhibition on the maturation of mast cells by coculture with Swiss 3T3 fibroblasts. (a)-(b) BMMCs were cultured for 16 days under three conditions: (1) BMMCs were cultured with IL-3 (1 ng/mL) and SCF (100 ng/mL), (2) BMMCs were cocultured with Swiss 3T3 fibroblasts in the presence of SCF (100 ng/mL) and anti-Wnt5a antibody (1 μg/mL), and (3) BMMCs were cocultured with Swiss 3T3 fibroblasts in the presence of SCF (100 ng/mL) and normal rat IgG control (1 μg/mL). (a) Suspensions of mast cells were stained with fluorescence-labeled anti-FcεRI, anti-c-kit, and anti-CD81 antibodies for 30 min on ice. Stained cells were washed, resuspended in 1% FBS-PBS, and analyzed by flow cytometry. Surface marker expression was analyzed by gating on viable cells in the FSC/SSC. One representative dot plot out of three independent experiments is shown. (b) Granule protease activities of mast cells were measured. All data represent the means ± SD (). .

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