Methylation Status of SP1 Sites within miR-23a-27a-24-2 Promoter Region Influences Laryngeal Cancer Cell Proliferation and Apoptosis

Figure 1

Effects of DNA methylation status of miR-23a-27a-24-2 cluster promoter CG-rich region on the cluster expression. (a) Prediction of miR-23a-27a-24-2 cluster promoter CG-rich region. CG-rich region spanning two SP1 sites is located at the cluster promoter, −530~−410. (b) DNA methylation status of the CG-rich region in Hep2 and HEK-293 cells. Cytosines of CG dinucleotides in the two SP1 sites were hypermethylated in HEK-293 cells compared to Hep2 cells. (c) Expression of miR-23a-27a-24-2 cluster in Hep2 and HEK-293 cells. Members of the cluster were upregulated in Hep2 cells compared to HEK-293 cells. (d) DNA methylation status of the CG-rich region in SAM-treated and SAM-untreated Hep2 cells. Cytosines of CG dinucleotides in the two SP1 sites were remethylated in SAM-treated Hep2 cells compared to SAM-untreated cells. (e) Expression of miR-23a-27a-24-2 cluster in SAM-treated and SAM-untreated Hep2 cells. Members of the cluster were downregulated in SAM-treated Hep2 cells compared to SAM-untreated cells. Hep2 cells were incubated with 1 mmol/L SAM. SAM-untreated cells were used as controls. indicates .

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