Improved Stability of a Model IgG3 by DoE-Based Evaluation of Buffer Formulations

Figure 1

Quantile graphs of the buffer formulations at all measurement points. (a) Recorded absorbance of the samples at 410 nm and (b) the percent aggregate as determined by SEC. Histidine formulations showed gross precipitation so large that they are captured by a SEC-column frit during analysis and this may have led to a false negative of percent aggregates (see Section 3.1.2). T0 denotes initial time point, T30 denotes 30-day storage time point, and F/T denotes freeze/thaw.

(a)

(b)