Improved Stability of a Model IgG3 by DoE-Based Evaluation of Buffer Formulations

Table 1

Single buffer DoE composition ranges. Levels for the individual buffer 2³ full factorial DoEs with center points.Each variable was assigned a high, middle, and low range before the full factorial was designed.

Buffer	Concentration (mM)	pH	NaCl (mM)
Acetate	25, 50, 100	4.5, 4.75, 5.0	25, 50, 100
Arginine	100, 200, 300	7.75, 8.0, 8.25
Histidine	25, 50, 100	6.25, 6.5, 6.75