Early Administration of Glutamine Protects Cardiomyocytes from Post-Cardiac Arrest Acidosis

Figure 3

Assessments of both the efficiency of cardiomyocyte transdifferentiation from iPSCs and the outcomes of low pH exposure. (a) Flow cytometry analysis showing that the differentiation efficiency of cTnT+ cells was 85.2%. (b–d) Cardiomyocytes with positive immunostaining for cTnT, NKX2.5, and DAPI at different magnifications. Scale bars: 100 um. (e) RT-PCR results showing that cardiomyocytes treated with 50 mM L-glutamine exhibited decreased expression of caspase-3, Bcl-xL, and p53 in both pH 6.5 and pH 7.3 HBSS buffers. These findings suggest that L-glutamine might protect cardiomyocytes from apoptosis caused by acidosis. Cell groups: A (normal culture medium), B (pH 6.5 HBSS buffer), C (pH 6.5 HBSS buffer plus 50 mM L-glutamine), D (pH 7.3 HBSS buffer), and E (pH 7.3 HBSS buffer plus 50 mM L-glutamine).

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