Syntaxin 5 Overexpression and β-Amyloid 1–42 Accumulation in Endoplasmic Reticulum of Hippocampal Cells in Rat Brain Induced by Ozone Exposure

Figure 2

Immunodetection of βA42 and Syx5 in the ER protein extracts of rat hippocampi following different O₃ treatments. (a) WB for βA42 in the ER fractions. The ER marker GRP78 was used as loading control and ER isolation control. (b) Densitometry analyses of the WB assays for βA42. βA42 production increased by the end of the ozone treatment. The differences became statistically significant at 90 days of O₃ exposure for βA42; . (c) WB for Syntaxin 5 from the ER fraction (control, 15, 30, 60, and 90 days). Rabbit anti-Syntaxin 5 was used for immunodetection and was visualized by chemiluminescence. (d) Densitometry analyses of the WB assays. There were significant changes in Syntaxin 5 expression at 60 and 90 days of treatment. The differences between the control and experimental groups were statistically significant; .