Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China
Table 3
Fragment lengths (base pairs) of the isolates with the patterns using the PRA-rpoB and PRA-hsp65 methods.
Number
Species
-rpoB
-hsp65
Species ()
Total
MspI
HaeIII
BstpI
HaeIII
1
M. abscessus
105, 95, 80
135, 100, 90
230, 210
140, 70, 60, 50
M. abscessus (44)
44
2
M. avium
105, 80, 45, 40
270
230, 210
130, 100, 60
M. avium (73)
73
3
M. chelonae
105, 95, 80
130, 100, 90
230, 210
200, 70, 60, 50
M. chelonae (1)
1
4
M. fortuitum
175, 100, 70
125, 85, 80
230, 120, 80
140, 120, 60, 55
M. fortuitum (8)
8
5
145, 95, 40
200, 90
230, 120, 95
145, 110, 40
M. gordonae (2)
12
145, 95, 40
210, 95, 90
235, 120, 85
160, 115, 60
M. gordonae (4)
175, 80, 40
270
230, 120, 80
160, 110, 60
M. gordonae (6)
6
M. holsaticum
105, 95, 90, 45
215, 85, 40
440
140, 100, 60, 50
M. holsaticum (1)
1
7
175, 100, 80
180, 90
230, 120, 95
150, 125, 60, 40
M. intracellulare (105)
154
180, 90, 80
185, 105
240, 120, 100
140, 125, 60, 40
M. intracellulare (2)
180, 90
180, 90, 80
240, 120, 100
140, 125, 60, 40
M. intracellulare (47)
8
Formerly M. massiliense
100, 90, 80, 75
125, 95, 85
235, 210
200, 70, 60, 50
Formerly M. massiliense (4)
4
9
M. monacense
359
210, 90
235, 130, 85
160, 90, 60
M. monacense (1)
1
10
M. neoaurum
140, 95, 40
200, 80
300, 115
170, 140, 40
M. neoaurum (1)
1
11
M. seoulense
120, 78, 70
200, 100
235, 210
145, 130
M. seoulense (1)
1
12
M. shimoidei
160, 100, 40
200, 70
240, 140, 80
140, 100, 70, 40
M. shimoidei (1)
1
13
M. szulgai
180, 80, 40
200, 120
440
125, 110, 45
M. szulgai (1)
1
14
Not identified
—
—
—
—
—
221
Different types of M. gordonae showed different patterns that need more research in the future. M. intracellulare displayed the different patterns in our study. Number of fragment lengths that are not identified. PRA: PCR-restriction fragment-length polymorphism analysis.
