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BioMed Research International
Volume 2016, Article ID 2414809, 11 pages
Research Article

Fibronectin-Grafted Titanium Dental Implants: An In Vivo Study

1School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan
2Graduate Institute of Biomedical Materials & Tissue Engineering, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan
3Department of International Logistics, Chung-Ang University, Seoul 156756, Republic of Korea
4Dental Department, Taipei Medical University Hospital, Taipei 110, Taiwan
5Department of General Dentistry, Chang Gung Memorial Hospital, Taipei 105, Taiwan
6Graduate Institute of Dental & Craniofacial Science, Chang Gung University, Taoyuan 333, Taiwan
7Dental Department, Taipei Medical University, Shuang-Ho Hospital, Taipei 235, Taiwan

Received 13 January 2016; Revised 23 March 2016; Accepted 9 May 2016

Academic Editor: Sandra Rodil

Copyright © 2016 Yu-Chi Chang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Modification of the physiochemical properties of titanium surfaces using glow discharge plasma (GDP) and fibronectin coating has been shown to enhance the surface hydrophilicity, surface roughness, cell adhesion, migration, and proliferation. This in vivo study aimed to evaluate the bone integration efficacy of a biologically modified implant surface. Two different surface-modified implants (Ar-GDP and GDP-fib) were placed in the mandibular premolar area of six beagle dogs for 2–8 weeks. Three techniques [histologic evaluation, resonance frequency analysis (RFA), and microcomputed tomography (micro-CT) evaluation] were used to detect the implant stability and bone-implant contact. The implant stability quotient values of GDP-fib implants were significantly greater than the Ar-GDP implants at 2 and 4 weeks (). The bone volume/total volume ratio of GDP-fib implants was greater than the Ar-GDP implants in micro-CT evaluation. A high positive correlation was observed between RFA and micro-CT measurements. At 2 weeks, osteoblasts were seen to line the implant surface, and multinuclear osteoclasts could be seen on the surface of old parent bone. After 8 weeks, a majority of the space in the wound chamber appeared to be replaced by bone. Enhancement of the stability of biologically modified implants was proved by the results of RFA, micro-CT, and histological analysis. This enhanced stability may help fasten treatment and be clinically beneficial.