Effect of Static Load on the Nucleus Pulposus of Rabbit Intervertebral Disc Motion Segment in an Organ Culture

Table 1

Details for the primers for the target and reference genes used in quantitative real-time PCR.

Gene	Primers	Primer sequence	bp
GAPDH	tuzi-GAPDH-F	CGAGACACGATGGTGAAGGT	131
	tuzi-GAPDH-R	ATGTAGTGGAGGTCAATGAATGG
Tu-COL2A1	Tu-COL2A1-F	ATGGCGGCTTCCACTTCA	92
	Tu-COL2A1-R	CTCAGTGGACAGCAGGCG
Tu-agg	Tu-agg-F	TTACCACCTACCCTTCACCTG	90
	Tu-agg-R	TTCTTCTGTCCAAAGGTCCTG

GAPDH: glyceraldehyde-3-phosphate dehydrogenase; COL2A1: collagen II; agg: aggrecan; F: forward; R: reverse.