Antipsychotic Drugs Inhibit Platelet Aggregation via P2Y₁ and P2Y₁₂ Receptors

Figure 5

The effect of chronic APDs on P2Y₁ and P2Y₁₂ in platelets from rats. Chronic APD-treated PRPs were produced by treating rats with the indicated concentration of APDs as described in Section 2. (a) Platelets were harvested at day 28 and ADP-induced calcium responses were measured. The peak responses were compared with those in nonpretreated ADP platelets. value < 0.05 for cells treated with chronic APDs followed by ADP stimulation compared to control cells stimulated with ADP alone. (b) Isolated platelets were harvested at day 28 and then incubated with 10 μM forskolin and 10 μM ADP at 37°C. Cell lysates were prepared and the levels of cAMP were measured using an enzyme immunoassay. Data are represented as the mean ± SEM (). value < 0.05 for cells treated with chronic APDs followed by forskolin and ADP stimulation compared to control cells stimulated with forskolin and ADP alone.

(a)

(b)