Antipsychotic Drugs Inhibit Platelet Aggregation via P2Y₁ and P2Y₁₂ Receptors

Figure 6

The effect of P2 receptor antagonists on APDs-mediated P2Y₁ and P2Y₁₂ receptors signaling in human platelets. (a) PRPs were pretreated with 1 × 10⁻⁶ M APDs for 1 hour and then incubated with Fura-2AM at 37°C. Platelets were harvested and then treated with 1 mM A2P5P for 3 min. ADP was added and subsequent calcium responses were measured. value < 0.05 for the control group compared to control group treated with A2P5P. (b) PRPs were pretreated with 1 × 10⁻⁶ M APDs for 1 hour. Isolated platelets were then incubated with 10 μM forskolin, 30 μM clopidogrel, and 10 μM ADP at 37°C. Cell lysates were prepared and the levels of cAMP were measured using an enzyme immunoassay. Data are represented as the mean ± SEM (). value < 0.05 for the control group compared to control group treated with forskolin. value < 0.05 for the control group treated with forskolin and ADP compared to the control group treated with forskolin only. value < 0.05 for cells treated with clopidogrel followed by forskolin and ADP stimulation compared to cells stimulated with forskolin and ADP alone.

(a)

(b)