Dehydroabietic Acid Derivative QC4 Induces Gastric Cancer Cell Death via Oncosis and Apoptosis

Figure 4

QC4 induced oncosis and apoptosis in gastric cancer cells. (a) PD150606 and Z-VAD-FMK partially reversed QC4 induced cell death. SGC-7901 and MGC80-3 cells were incubated with PD150606, Z-VAD-FMK, or Necrostatin-1 at indicated concentrations for 24 h alone or in combination with QC4 administration at indicated concentrations for 24 h. Cell viability was calculated by CCK-8 assay. (b) QC4 activated oncosis marker calpain-1 autolysis from the 80 kDa event to the 76 kDa event. (c) QC4 degraded β-actin and α-tubulin. (d) Ultramicrostructure of SGC-7901 cells after 10 μg/mL QC4 administration for indicated time. (e) QC4 activated the cleavage of apoptotic proteins. Carboplatin (CBP) was set as positive control ( for the combination of QC4 with PD150506 treatment group against the QC4 alone treatment negative control group in SGC-7901; for the combination of QC4 with Z-VAD-FMK treatment group against the QC4 alone treatment negative control group in SGC-7901; for the combination of QC4 with PD150506 treatment group against the QC4 alone treatment negative control group in MGC80-3; for the combination of QC4 with Z-VAD-FMK treatment group against the QC4 alone treatment negative control group in MGC80-3).

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