Clinical and Histological Evaluation of Direct Pulp Capping on Human Pulp Tissue Using a Dentin Adhesive System

Figure 2

Human pulp capped with Single Bond Universal. (a) Incomplete dentin bridge formation and no inflammatory process visible in the remaining pulp (hematoxylin and eosin [H&E]). Scale bar = 400 μm. (a1) Fragment of the view seen in frame 1 of panel (a). The dentin bridge with tunnel defect (arrowhead) is observed. Scale bar = 50 μm. (a2) Fragment of the view seen in frame 2 of panel (a). A layer of the bonding system is observed under the tissue of the bridge. Scale bar = 50 μm. (b) No dentin bridge. Pulp fibrosis at the site of contact with the Single Bond Universal (H&E). Scale bar = 300 μm. (b1) Fragment of the view seen in panel (b). Tissue resembling fibrodentin. Scale bar = 50 μm. (c) No dentin bridge (H&E). Scale bar = 600 μm. (c1) Fragment of the view seen in frame 1 of panel (c). Hemorrhagic infiltration and presence of the material in the pulp. Scale bar = 350 μm. (c2) Fragment of the view seen in frame 2 of panel (c). Thinning of the odontoblast layer and tertiary dentin near the site of exposure. Scale bar = 150 μm. (d) No dentin bridge. Pulpitis with rich infiltrate of leukocytes (star) and marked dilation of blood vessels (H&E). Scale bar = 500 μm. (d1) Fragment of the view seen in panel (d) with amorphous areas of necrosis and rich infiltrate of leukocytes. Scale bar = 100 μm. (e) No dentin bridge. Chronic pulpitis (H&E). Scale bar = 100 μm. (e1) View of the preparation stained according to Brown and Brenn (B&B). No positive staining for bacteria. Scale bar = 100 μm. D: dentin; DB: dentin bridge; OL: odontoblast layer; P: pulp; TD: tertiary dentin; arrowhead: tunnel defect; star: infiltrate of leukocytes.