Using Small RNA Deep Sequencing Data to Detect Human Viruses

Figure 1

Nucleotide polymorphism, hotspots, and siRNA duplexes of HIV-1. The -axis represents positions on the HIV-1 reference genome (GenBank: M19921). The -axis represents the read counts from the data SRR941591 on each position. The dots in the top black box represent positions with polymorphic nucleotides. #1, #2, and #3 are the size distributions of positive- and negative-strand viral reads in hotspot 1 (779–810 bp), hotspot 2 (2,017–2,045 bp), and hotspot 3 (12,006–12,044 bp). The read counts of 21 bp, 22 bp, 23 bp, and 24 bp siRNA duplexes are marked in parentheses.