Rapid and Quantitative Detection of Leifsonia xyli subsp. xyli in Sugarcane Stalk Juice Using a Real-Time Fluorescent (TaqMan) PCR Assay

Figure 2

Specificity of real-time qPCR with TaqMan probe using individual DNA templates, total DNA (100 ng) of Lxx- (Leifsonia xyli subsp. xyli-) infected stalk juice, bacterial genomic DNA (100 pg) of Lxx, L. ginsengi, L. poae, and L. rubra, and total DNA (100 ng) of Lxx-free stalk juice (negative control) and sterile H₂O (blank control). Three technical replicates were used in each DNA concentration.