Rapid and Quantitative Detection of Leifsonia xyli subsp. xyli in Sugarcane Stalk Juice Using a Real-Time Fluorescent (TaqMan) PCR Assay

Table 2

Detection of Leifsonia xyli subsp. xyli (Lxx) in stalk juice samples from sugarcane fields.

Trial	Variety	Conventional PCR		qPCR
		CxxITSf#5/CxxITSr#5	Pat1-F2/Pat1-R2	Pat1-QF/Pat1-QR	Concentration of DNA (copies/µL)
Trial #10	FN07-2020	1/6	1/6	1/6	7.65 × 10^2
	FN07-3206	0/6	0/6	0/6	na
	FN40	1/6	1/6	1/6	2.19 × 10^2
	GZ07-538	0/6	0/6	1/6	1.90 × 10^2
	LC07-500	0/6	0/6	0/6	na
	LC07-536	0/6	0/6	1/6	1.42 × 10^2
	MT02-205	0/6	0/6	2/6	1.20 × 10^2–1.85 × 10^2
	ROC16	4/6	3/6	5/6	2.40 × 10^2–4.02 × 10^2
	ROC22	0/6	0/6	0/6	na
	YG43	0/6	0/6	1/6	1.94 × 10^2
	YG46	1/6	3/6	4/6	2.92 × 10^2–1.11 × 10^3
	YR07-1433	0/6	0/6	0/6	na
	YZ07-2384	0/6	0/6	0/6	na
	YZ08-2060	0/6	0/6	1/6	1.62 × 10^2
Trial #11	DHZ07-36	0/6	0/6	1/6	1.16 × 10^2
	FN09-0906	0/6	0/6	1/6	2.13 × 10^3
	FN09-7111	0/6	2/6	3/6	2.98 × 10^2–2.11× 10^4
	GT06-2081	0/6	0/6	1/6	4.04 × 10^2
	GT07-25	0/6	1/6	2/6	4.11 × 10^2–1.75 × 10^4
	GT08-1180	0/6	0/6	1/6	1.59 × 10^2
	HZ22	0/6	0/6	0/6	na
	LC07-150	0/6	1/6	2/6	1.23 × 10^3–1.29 × 10^3
	MT06-1045	3/6	4/6	4/6	1.09 × 10^2–5.47 × 10^3
	ROC22	0/6	0/6	1/6	5.61 × 10^2
	YG45	4/6	4/6	5/6	1.73 × 10^2–5.54 × 10^3
	YG47	6/6	6/6	6/6	1.28 × 10^2–1.16 × 10^4
	YR06-241	0/6	0/6	3/6	1.15 × 10^2–1.98 × 10^3
	YZ08-1095	0/6	0/6	1/6	2.94 × 10^3
	YZ08-1145	1/6	1/6	2/6	4.21 × 10^2–3.75 × 10^3

na, data not available.