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BioMed Research International
Volume 2016, Article ID 2742648, 9 pages
http://dx.doi.org/10.1155/2016/2742648
Research Article

Previously Unidentified Single Nucleotide Polymorphisms in HIV/AIDS Cases Associate with Clinical Parameters and Disease Progression

1Kazan State Medical University, 49 Butlerova St., Kazan 420012, Russia
2Republican Clinical Hospital for Infectious Disease named after Agafonov, 83 Prospect Pobedy St, Kazan 420140, Russia
3Republican Center for the Prevention and Fighting of AIDS and Infectious Diseases, Ministry of Health of the Republic of Tatarstan, 2a Vishevskogo St., Kazan 420097, Russia
4Kazan Federal University, 18 Kremlyovskaya St., Kazan 420008, Russia
5Department of Biochemistry, University of Nevada, Reno, 1664 N. Virginia Street MS 0330, Reno, NV 89557, USA
6Nevada Center for Biomedical Research, University of Nevada, Reno, 1664 N. Virginia Street MS 0552, Reno, NV 89557, USA

Received 19 August 2016; Accepted 8 November 2016

Academic Editor: Guochun Jiang

Copyright © 2016 Vladimir V. Anokhin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The genetic background of an individual plays an important role in the progression of HIV infection to AIDS. Identifying previously unknown or uncharacterized single nucleotide polymorphisms (SNPs) that associate with disease progression may reveal important therapeutic targets and provide a greater understanding of disease pathogenesis. In the present study, we employed ultra-high multiplex PCR on an Ion Torrent next-generation sequencing platform to sequence 23 innate immune genes from 94 individuals with HIV/AIDS. This data was used to identify potential associations of SNPs with clinical parameters and disease progression. SNPs that associated with an increased viral load were identified in the genes for the interleukin 15 receptor (IL15RA), toll-like receptor 7 (TLR7), tripartite motif-containing protein 5 (TRIM5), and two killer-cell immunoglobulin-like receptors (KIR2DL1 and KIR2DL3). Additionally, SNPs that associated with progression from HIV infection to AIDS were identified in two 2′-5′-oligoadenylate synthetase genes (OAS2 and OAS3). In contrast, other SNPs identified in OAS2 and OAS3 genes, as well as in the TRIM5 and KIR2DS4 genes, were associated with a slower progression of disease. Taken together, our data demonstrates the utility of ultra-high multiplex PCR in identifying polymorphisms of potential clinical significance and further,identifies SNPs that may play a role in HIV pathogenesis.