Proteome Changes of Human Bone Marrow Mesenchymal Stem Cells Induced by 1,4-Benzoquinone

Table 1

Differentially expressed proteins in 1,4-BQ treated hBM-MSCs identified by LC-MS/.

Spot	Protein name	UniProt ID	Function	Unique peptide count	Theoretical MW/pI	Ratio of spot volume (-value)
						10 μM	25 μM	50 μM
1878	Alpha-actin-1	P68133	Involved in various types of cell motility	2	42.1/5.23	(0.0090)		(0.0001)
1874	Beta-actin	P60709	Involved in various types of cell motility	3	41.7/5.29	(0.0062)	(0.0016)	(0.0002)
1997	Gelsolin	P06396	Actin-modulating protein	3	85.7/5.90	(0.0028)
2550	Creatine kinase M-type (M-CK)	P06732	Creatine metabolic process	2	43.1/6.77	(0.0055)
2665	Beta-enolase	P13929	Glycolysis	2	47.0/7.58	1.7390 (0.0080)
4590	Transcription elongation factor B polypeptide 1	Q15369	Transcription regulation	1	12.5/4.74		(0.0033)
4905	Isoform 1 of 26S proteasome non-ATPase regulatory subunit 1	Q99460-1	Degradation of proteins	2	105.8/5.25		(0.0251)
5581	Ragulator complex protein LAMTOR2	Q9Y2Q5	Signal transduction	2	13.5/5.30		(0.0132)
4027	Isoform 4 of Crumbs homolog 1 precursor	P82279-4	Cell-cell signaling	1	104.2/5.45		(0.0081)
4644	Translocon-associated protein subunit delta	P51571	Intracellular protein transport	5	20.2/5.53		(0.0001)
5261	TAR DNA-binding protein 43	Q13148	transcription regulation	5	44.7/5.85		1.6949 (0.0095)
4467	Isoform Short of Proteasome subunit alpha type 1	P25786-1	Immunity	11	29.6/6.15		1.6421 (0.0024)
4414	Ribose-phosphate pyrophosphokinase 2	P11908	Nucleotide biosynthesis	1	34.8/6.15		2.1110 (0.0023)
4343	Apolipoprotein L2	Q9BQE5	Lipid transport	4	37.1/6.28		1.8877 (0.0008)
4336	Transaldolase	P37837	Energy reserve metabolic process	4	37.5/6.36		1.5618 (0.0050)
4186	Lupus La protein	P05455	tRNA modification	2	46.8/6.68		1.6213 (0.0011)
4493	26S proteasome non-ATPase regulatory subunit 8 (PSMD8)	P48556	Degradation of proteins	2	32.6/6.85		1.5107 (0.0102)
4605	Calponin-2	Q99439	Regulation of actin filament-based process	1	33.7/6.94		(0.0000)
4551	Glutathione S-transferase M1 isoform 2	P09488	Glutathione transferase activity	2	21.3/7.67		1.9390 (0.0193)
3604	Heat shock protein 90 kDa beta member 1	P14625	Molecular chaperone that functions in the processing and transport of secreted proteins	2	92.5/4.76			(0.0101)
1553	Vimentin	P08670	Structural constituent of cytoskeleton	8	53.7/5.06			2.0727 (0.0015)
1070	Heat shock 70 kDa protein 5	P11021	Facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum	3	72.4/5.07			2.7257 (0.0022)
1339	Rap1 GTPase-GDP dissociation stimulator 1	P52306	Stimulating GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins)	2	66.3/5.21			(0.0109)
1166	Heat shock cognate 71 kDa protein	P11142-1	Acting as a repressor of transcriptional activation	2	70.9/5.37			1.5630 (0.0144)
1235	Copine-1	Q99829	Membrane trafficking	3	59.1/5.52			(0.0039)
1290	Copine-3	O75131	Membrane trafficking	3	60.1/5.60			(0.0022)
1013	Collagen alpha-1(I) chain	P02452	Leukocyte migration. Platelet activation	5	138.9/5.60			1.8513 (0.0071)
2035	Superoxide dismutase [Cu-Zn]	P00441	Destroying radicals	2	15.9/5.70			1.9264 (0.0050)
2015	Ferritin	Q6S4P3	Cellular iron ion homeostasis	1	21.3/5.70			2.0167 (0.0001)
1232	Heat shock protein 60	P10809	Preventing misfolding under stress conditions in the mitochondrial matrix	1	61.1/5.70			(0.0180)
1983	Putative uncharacterized protein DKFZp686N1815	Q5HYI3	Proteolysis	1	17.6/5.83			1.6203 (0.0389)
1915	Heat shock 27 kDa protein	P04792	Involved in stress resistance and actin organization	4	22.8/5.98			(0.0014)

of spot volume means the spot intensity ratio of treatment group to control group (>1.5, increased in treatment group; <1.5, decreased in treatment group). Theoretical molecular weights (MW in kDa) and isoelectric points (pI) were determined from database sequence entries. Note that the theoretical values do not account for potential posttranslational modification.