Effect of Phosphatase and Tensin Homologue on Chromosome 10 on Angiotensin II-Mediated Proliferation, Collagen Synthesis, and Akt/P27 Signaling in Neonatal Rat Cardiac Fibroblasts

Figure 4

Overexpression of PTEN inhibits Ang II-induced collagen synthesis in CFs. After infection for 48 h, fibroblasts were treated with Ang II (10⁻⁷ mM) for 32 h. (a, b) The collagen type I-α1 mRNA level was assessed by semiquantitative RT-PCR. (c, d) The collagen type III-α1 mRNA level was assessed by semiquantitative RT-PCR. (e) The protein synthesis rate of collagen was assessed by ³H-proline incorporation assay. Each experiment was repeated three times with 3-4 replicates. , compared with the control Ad-GFP and untreated groups. , compared with Ad-GFP + Ang II.

(a)

(b)

(c)

(d)

(e)