Effect of Phosphatase and Tensin Homologue on Chromosome 10 on Angiotensin II-Mediated Proliferation, Collagen Synthesis, and Akt/P27 Signaling in Neonatal Rat Cardiac Fibroblasts

Figure 5

Overexpression of PTEN inhibits Ang II-induced MMP-2 and TIMP-1 production in CFs. After infection for 48 h, fibroblasts were treated with Ang II (10⁻⁷ mM) for 32 h. (a, b) The MMP-2 mRNA level was assessed by RT-PCR. (c, d) The TIMP-1 mRNA level was assessed by RT-PCR. (e, f) The activity of gelatinase was assessed by gelatin zymography. Each experiment was repeated three times with 3-4 replicates. , compared with the control Ad-GFP-infected and uninfected cells. , compared with Ad-GFP + Ang II.

(a)  

(b)

(c)

(d)

(e)

(f)