Effect of Phosphatase and Tensin Homologue on Chromosome 10 on Angiotensin II-Mediated Proliferation, Collagen Synthesis, and Akt/P27 Signaling in Neonatal Rat Cardiac Fibroblasts

Table 1

Primers designed and used for the RT-PCR studies.

Gene	Primers	Product size (bp)	Annealing temperature
PTEN	Forward 5′-AGAACTTATCAAACCCTT-3′	186	55°C
	Reverse 5′-GTCCTTACTTCCCCAT-3′
Col I-1	Forward 5′-CTCAGGGGCGAAGGCAACAGT-3′	125	50°C
	Reverse 5′-ATGGGCAGGCGGGAGGTCT-3′
Col III-1	Forward 5′-ATGGTGGCTTTCAGTTCAGC-3′	425	45°C
	Reverse 5′-TGGGGTTTCAGAGAGTTTGG-3′
MMP-2	Forward 5′-TGG TCGCAGTGATGGCTTCCTCT-3′	414	57°C
	Reverse 5′-CCCCACTTCCGGTCATCATCGTAG-3′
TIMP-1	Forward 5′-TCGACGCTGTGGGGAATG-3′	466	54°C
	Reverse 5′-AAAGAACGGAGGAAACAG-3′
GAPDH	Forward 5′-TGCTGAGTATGTCGTGGAGT-3′	289	55°C
	Reverse 5′-AGTCTTCTGAGTGGCAGTGAT-3′