Optimized Collection Protocol for Plasma MicroRNA Measurement in Patients with Cardiovascular Disease

Figure 5

Heparinase I treatment improved Ct values of qPCR signals of clinical samples. (a) The qPCR signal was enhanced by treatment with 0.5 U and 0.25 U of heparinase I in spike-in control cel-miR-39 in four individual plasma samples stored for 0.5 h (top) or at 8 h (bottom) at RT or at 4°C. (b) Two individual plasma samples (P03 ad P04) treated with 0.5 U and 0.25 U heparinase I showed similar qPCR detection results for four independent miRNA targets. (c) Heparinase I treatment improved the qPCR signal. For cel-miR-39 (top), a dose-dependent effect of heparinase I was obsercved in samples stored for 2 h or 4 h at RT or at 4°C. Similar results were observed in four individual samples of human miR-15b-5p. (d) A heparinase I dose of 0.25 U significantly improved the qPCR signal in samples stored for 2 h or 4 h at RT or at 4°C in one-way ANOVA. ;   ;   .

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