Enhancement of Polymerase Activity of the Large Fragment in DNA Polymerase I from<i> Geobacillus stearothermophilus</i> by Site-Directed Mutagenesis at the Active Site

<div>Identification of recombinant plasmids by colony PCR. (a) M: 2 kb ladder marker; 1: WT of Bst DNA pol LF gene; (b) M: 10 kb ladder marker; 1: WT of Bst DNA pol LF gene; 2: LF mutant D540A; 3: LF mutant D540E; 4: LF mutant G310A; 5: LF mutant G310L; 6: LF mutant R412A; 7: LF mutant R412E; 8: LF mutant K416A; 9: LF mutant K416D; 10: LF mutant G310A-D540E; 11: LF mutant G310L-D540E.</div>

BioMed Research International

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Figure 1

Figure 1: Enhancement of Polymerase Activity of the Large Fragment in DNA Polymerase I from<i> Geobacillus stearothermophilus</i> by Site-Directed Mutagenesis at the Active Site 

Figure 1 | Enhancement of Polymerase Activity of the Large Fragment in DNA Polymerase I from Geobacillus stearothermophilus by Site-Directed Mutagenesis at the Active Site 