Research Article

Enhancement of Polymerase Activity of the Large Fragment in DNA Polymerase I from Geobacillus stearothermophilus by Site-Directed Mutagenesis at the Active Site

Figure 2

SDS-PAGE analysis of the WT of Bst DNA pol LF. M: protein ladder marker shown in kDa on the left sides of panels; 1: uninduced whole cell sample; 2: supernatant fraction of uninduced sample; 3: pellet fraction of uninduced sample; 4: whole cell sample after induction for 6 h; 5: supernatant fraction after induction for 6 h; 6: pellet fraction after induction for 6 h. Corresponding position of Bst DNA pol LF was marked by black arrow.