Phosphoprotein Phosphatase 1 Is Required for Extracellular Calcium-Induced Keratinocyte Differentiation

Figure 2

Knockdown of PP1 blocks calcium-induced PIP5K1α dephosphorylation. Cultured human keratinocytes were treated with PP1α siRNA, PP1β siRNA, and PP1γ siRNA for 72 hours and then with calcium for 5 min. (a) Cells were harvested and protein levels of PP1α, PP1β, and PP1γ were determined by western analysis. (b) Data expressed are mean ± SD of three separate experiments, .

(a)

(b)